Please look at some examples of our efforts:

Fig. 1. Human non-tagged ubiquitin, final elution from heparin sepharose

Fig. 2. Artificial mini-intein purified on the Ni-NTA sepharose.

• m - molecular size markers
• 1 to 5 - elution fractions
• 12.5 % PAAG, Comassie Blue staining

• t - total bacterial cell lysate
• ft - flowthough
• w- washing
• m - molecular size markers
• elut 1 to 7 - elution fractions of the final preparation

10 % PAAG, Comassie Blue staining

The homogeneity of proteins is checked by the SDS-PAGE stained with Coumassie blue and scanned by gel scanner.

Two grades of protein purity are available:

- standard (>85% of homogeneity)

- extra pure (>96% of homogeneity)